Discussion on the Construction Strategy of WeChat Public Platform Based on Ideological and Political Education in Colleges and Universities

WeChat transmission has the characteristics of fast transmission speed, strong content selectivity and wide spread. The construction of WeChat public platform based on the ideological and political education in colleges and universities is a work that cannot be ignored in the ideological and political education, which is the supplement and extension of the practical teaching of ideological and political courses in colleges and universities, and can significantly enhance the pertinence and effectiveness of ideological and political education. The construction of WeChat public platform based on ideological and political education in colleges and universities should stick to the core idea of platform construction, define the stage target of the construction, build a professional team of WeChat public platform operation, improve the operation mechanism, integrate the resources of WeChat public platform construction, carefully consider the details of WeChat public platform operation, and collect feedback information and respond effectively, so as to improve the construction quality of WeChat public platform and enhance the transmission effect. In view of the deficiencies in the construction of WeChat public platform for ideological and political education in colleges and universities, measures should be taken to improve the communication power, influence, guidance and credibility of WeChat public platform, so that WeChat public platform can give full play to its value orientation and knowledge dissemination role in ideological and political education in colleges and universities

Mechanisms of Chloroperoxidases-Catalyzed Enantioselective Transformations from Spectroscopic and X-ray Crystallographic Studies of Enzyme-substrate Complexes

The chloroperoxidase secreted from Caldariomyces fumago catalyzes broad spectrum of reactions. The crystallography combined with X-ray diffraction analysis was conducted to reveal recombinant CPO expressed in a modified Aspergillus niger system. Our results indicated that despite functional similarities with wild type CPO, recombinant CPO is over glycosylated with more mannose. Besides, ten iodide ion binding sites were identified in rCPO and six of them were found to be well superimposed on previously reported structure of the wild type CPO. Therefore, recombinant CPO shares almost the same structure with wild type CPO, and the Aspergillus niger is a potential system for heterologous heme peroxidase expression. The most striking feature of C. fumago chloroperoxidase is its ability to efficiently catalyze asymmetric epoxidations of alkenes with excellent enantioselectivity. To elucidate the structural basis for the mechanism of CPO-catalyzed enantioselective epoxidation of selected olefins, the structures of the CPO and its olefin complex were studied by both crystallographic and computational techniques. Molecular docking studies demonstrated that ethyl 3-methylbut-3-enoate forms a complex with CPO at a relatively flexible channel through hydrogen bond interactions with Ala 75, coupled with other Pi-interactions between Phe186 and the carbon-carbon double bond of the substrate, ensuring its enantioselective transformation. CPO mutants of C29H, F186A and F103A/186A were expressed in A. niger and results of catalytic assays dwarfed the effects of thiolate-ligand, while confirming the roles of Phe103 and Phe186 in enzyme-substrate interactions and catalytic activities. The prokaryotic system was applied to express mutant CPO-C29H. Our work demonstrated that the heme binding of C29H is improved with the assistance of ChuA gene, but C29H expressed in E. coli cells is inactive in either chlorination or peroxidation, indicating the requirement of post-translational modifications for mutant CPO

Dynamic Reliability Assessment of Heavy Vehicle Crossing a Prototype Bridge Deck by Using Simulation Technology and Health Monitoring Data

Overloads of vehicle may cause damage to bridge structures, and how to assess the safety influence of heavy vehicles crossing the prototype bridge is one of the challenges. In this report, using a large amount of monitored data collected from the structural health monitoring system (SHMS) in service of the prototype bridge, of which the bridge type is large-span continuous rigid frame bridge, and adopting FEM simulation technique, we suggested a dynamic reliability assessment method in the report to assess the safety impact of heavy vehicles on the prototype bridge during operation. In the first place, by using the health monitored strain data, of which the selected monitored data time range is before the opening of traffic, the quasi dynamic reliability around the embedded sensor with no traffic load effects is obtained; then, with FEM technology, the FEM simulation model of one main span of the prototype bridge is built by using ANSYS software and then the dynamic reliability when the heavy vehicles crossing the prototype bridge corresponding to the middle-span web plate is comprehensively analyzed and discussed. At last, assuming that the main beam stress state change is in the stage of approximately linear elasticity under heavy vehicle loads impact, the authors got the impact level of heavy vehicles effects on the dynamic reliability of the prototype bridge. Based on a large number of field measured data, the dynamic reliability value calculated by our proposed methodology is more accurate. The method suggested in the paper can do good for not only the traffic management but also the damage analysis of bridges